ABSTRACT
This article describes the progress of developing the training base and training methods for bronchoscopists at Changhai hospital in recent years,and then discusses the potential issues and solutions that might occure in the course of training,and finally explores the model and methodology to optimize the training program for Chinese bronchosocpists.
ABSTRACT
Objective To explore the effects of antisense vector of annexinⅡ gene on the growth of SPC A 1, a human lung cancer cell line. Methods The total RNA was isolated from human lung cancer cell line SPC A 1 and the target DNA fragments were amplified by RT PCR. The antisense expression vector was constructed by double restriction endonuclease cleavage directional clone method. Annexin Ⅱ antisense expression vector was introduced into SPC A 1 cells by liposome transfection reagent. The expression of annexin Ⅱ mRNA was analyzed by semi quantitative RT PCR. The effects of antisense vector of annexinⅡ gene on the growth of SPC A 1 were observed. Results The antisense vector of annexinⅡ gene was constructed and introduced into SPC A 1 cells successfully. Semi quantitative RT PCR showed that the annexin Ⅱ mRNA expression reduced by about two thirds in the transfected cells as compared with that in the untransfected cells. Compared with the untransfected cells, transfected cells decreased significantly in cell growth, clone formation efficiency in plating and DNA synthesis. Cell cycle was blocked in G 0 G 1 phase. Conclusion Annexin Ⅱ could promote the growth of lung cancer cells and may be helpful for the development of lung cancer.
ABSTRACT
Objective To analyze the effects of annexin Ⅱ antisense expression vector on the growth of the transplanted tumor in nude mice. Methods The SPC-A-1 cells (parental group) and SPC-A-1-annexin Ⅱ cells (antisense group) were inoculated subcutaneously in nude mice respectively. The forming time, volume, and weight of tumor were measured. The tumor cells and the surrounding tissues were observed microscopically. Results The forming time of tumor in the antisense group was later than that in the parental group. The volume and weight of tumor in the antisense group were lower than those in the parental group. Furthermore, invasion of tumor in the surrounding tissues was found in 2 cases in the parental group, but not in the antisense group. Conclusion The annexin Ⅱ gene may be important in promoting tumor cell growth and invading the surrounding tissues.